Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1500 pg/ml | 3.052 | 2.911 | 2.779 |
2.77 | |||
750 pg/ml | 1.69 | 1.684 | 1.552 |
1.678 | |||
375 pg/ml | 0.914 | 0.935 | 0.803 |
0.957 | |||
187.5 pg/ml | 0.588 | 0.556 | 0.424 |
0.524 | |||
93.75 pg/ml | 0.338 | 0.36 | 0.228 |
0.382 | |||
46.875 pg/ml | 0.259 | 0.248 | 0.116 |
0.238 | |||
23.4375 pg/ml | 0.183 | 0.19 | 0.058 |
0.197 | |||
0 pg/ml | 0.125 | 0.132 | 0 |
0.139 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 188 | 19.2 | 10.2 |
Intra-assay | 2 | 20 | 460 | 16.5 | 3.6 |
Intra-assay | 3 | 20 | 1179 | 40.9 | 3.5 |
Inter-assay | 1 | 20 | 187 | 17.6 | 9.4 |
Inter-assay | 2 | 20 | 464 | 36.1 | 7.8 |
Inter-assay | 3 | 20 | 1177 | 94.8 | 8.1 |
Protocols
References
Customer reviews and Q&As