Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1500 pg/ml | 2.316 | 2.427 | 2.346 |
2.538 | |||
750 pg/ml | 1.249 | 1.25 | 1.169 |
1.25 | |||
375 pg/ml | 0.625 | 0.652 | 0.571 |
0.678 | |||
187.5 pg/ml | 0.37 | 0.364 | 0.283 |
0.357 | |||
93.75 pg/ml | 0.206 | 0.219 | 0.138 |
0.231 | |||
46.875 pg/ml | 0.135 | 0.143 | 0.062 |
0.15 | |||
23.4375 pg/ml | 0.109 | 0.107 | 0.026 |
0.104 | |||
0 pg/ml | 0.077 | 0.081 | 0 |
0.084 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 264 | 6.8 | 2.6 |
Intra-assay | 2 | 20 | 575 | 22.7 | 3.9 |
Intra-assay | 3 | 20 | 1173 | 46 | 3.9 |
Inter-assay | 1 | 20 | 264 | 21.5 | 8.2 |
Inter-assay | 2 | 20 | 578 | 46.4 | 8 |
Inter-assay | 3 | 20 | 1175 | 69.8 | 5.9 |
Protocols
References
Customer reviews and Q&As