Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1200 pg/ml | 2.818 | 2.709 | 2.625 |
2.601 | |||
600 pg/ml | 1.477 | 1.442 | 1.358 |
1.407 | |||
300 pg/ml | 0.808 | 0.802 | 0.718 |
0.796 | |||
150 pg/ml | 0.425 | 0.445 | 0.361 |
0.465 | |||
75 pg/ml | 0.278 | 0.264 | 0.18 |
0.251 | |||
37.5 pg/ml | 0.171 | 0.17 | 0.086 |
0.169 | |||
18.75 pg/ml | 0.121 | 0.129 | 0.045 |
0.138 | |||
0 pg/ml | 0.087 | 0.084 | 0 |
0.082 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 91.2 | 3.2 | 3.5 |
Intra-assay | 2 | 20 | 268 | 7.9 | 3 |
Intra-assay | 3 | 20 | 654 | 19.1 | 2.9 |
Inter-assay | 1 | 20 | 92 | 7.6 | 8.2 |
Inter-assay | 2 | 20 | 270 | 22 | 8.2 |
Inter-assay | 3 | 20 | 655 | 31.9 | 4.9 |
Protocols
References
Customer reviews and Q&As