Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1000 pg/ml | 2.725 | 2.811 | 2.698 |
2.897 | |||
500 pg/ml | 1.608 | 1.602 | 1.489 |
1.595 | |||
250 pg/ml | 0.949 | 0.907 | 0.794 |
0.865 | |||
125 pg/ml | 0.512 | 0.507 | 0.394 |
0.502 | |||
62.5 pg/ml | 0.318 | 0.317 | 0.204 |
0.316 | |||
31.25 pg/ml | 0.215 | 0.205 | 0.092 |
0.195 | |||
15.625 pg/ml | 0.153 | 0.161 | 0.048 |
0.169 | |||
0 pg/ml | 0.114 | 0.113 | 0 |
0.112 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 88.5 | 5.2 | 5.9 |
Intra-assay | 2 | 20 | 221 | 9.9 | 4.5 |
Intra-assay | 3 | 20 | 503 | 21.9 | 4.4 |
Inter-assay | 1 | 20 | 89.3 | 8.9 | 9.9 |
Inter-assay | 2 | 20 | 222 | 16.1 | 7.3 |
Inter-assay | 3 | 20 | 501 | 31 | 6.2 |
Protocols
Product publications
Customer reviews and Q&As