Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1000 pg/ml | 2.635 | 2.517 | 2.408 |
2.399 | |||
500 pg/ml | 1.372 | 1.393 | 1.284 |
1.414 | |||
250 pg/ml | 0.8 | 0.773 | 0.664 |
0.745 | |||
125 pg/ml | 0.447 | 0.441 | 0.332 |
0.434 | |||
62.5 pg/ml | 0.273 | 0.279 | 0.17 |
0.284 | |||
31.25 pg/ml | 0.185 | 0.191 | 0.082 |
0.197 | |||
15.625 pg/ml | 0.165 | 0.156 | 0.047 |
0.147 | |||
0 pg/ml | 0.107 | 0.109 | 0 |
0.111 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 151 | 3.2 | 2.1 |
Intra-assay | 2 | 20 | 315 | 8.1 | 2.6 |
Intra-assay | 3 | 20 | 787 | 24.3 | 3.1 |
Inter-assay | 1 | 20 | 150 | 9.5 | 6.3 |
Inter-assay | 2 | 20 | 316 | 15.8 | 5 |
Inter-assay | 3 | 20 | 778 | 47 | 6 |
Protocols
References
Customer reviews and Q&As