Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
800 pg/ml | 2.371 | 2.432 | 2.322 |
2.494 | |||
400 pg/ml | 1.341 | 1.311 | 1.201 |
1.281 | |||
200 pg/ml | 0.695 | 0.726 | 0.616 |
0.758 | |||
100 pg/ml | 0.427 | 0.42 | 0.31 |
0.414 | |||
50 pg/ml | 0.256 | 0.268 | 0.158 |
0.281 | |||
25 pg/ml | 0.194 | 0.189 | 0.079 |
0.185 | |||
12.5 pg/ml | 0.158 | 0.152 | 0.042 |
0.146 | |||
0 pg/ml | 0.114 | 0.11 | 0 |
0.106 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 49.3 | 2 | 4 |
Intra-assay | 2 | 20 | 159 | 5.7 | 3.6 |
Intra-assay | 3 | 20 | 530 | 24.9 | 4.7 |
Inter-assay | 1 | 20 | 48.9 | 4 | 8.2 |
Inter-assay | 2 | 20 | 160 | 10.5 | 6.6 |
Inter-assay | 3 | 20 | 529 | 31.1 | 5.9 |
Protocols
Product publications
Customer reviews and Q&As