Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1000 pg/ml | 2.713 | 2.804 | 2.68 |
2.896 | |||
500 pg/ml | 1.356 | 1.388 | 1.264 |
1.42 | |||
250 pg/ml | 0.684 | 0.721 | 0.597 |
0.758 | |||
125 pg/ml | 0.431 | 0.41 | 0.286 |
0.389 | |||
62.5 pg/ml | 0.273 | 0.262 | 0.138 |
0.251 | |||
31.25 pg/ml | 0.177 | 0.189 | 0.065 |
0.201 | |||
15.625 pg/ml | 0.152 | 0.157 | 0.033 |
0.162 | |||
0 pg/ml | 0.127 | 0.124 | 0 |
0.121 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 76.8 | 5.3 | 6.9 |
Intra-assay | 2 | 20 | 174 | 8 | 4.6 |
Intra-assay | 3 | 20 | 349 | 13.2 | 3.8 |
Inter-assay | 1 | 20 | 76.2 | 6.7 | 8.8 |
Inter-assay | 2 | 20 | 176 | 9.6 | 5.4 |
Inter-assay | 3 | 20 | 351 | 24.7 | 7 |
Protocols
References
Customer reviews and Q&As