Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
500 pg/ml | 2.592 | 2.472 | 2.378 |
2.351 | |||
250 pg/ml | 1.346 | 1.294 | 1.2 |
1.241 | |||
125 pg/ml | 0.648 | 0.682 | 0.588 |
0.715 | |||
62.5 pg/ml | 0.393 | 0.385 | 0.291 |
0.376 | |||
31.25 pg/ml | 0.232 | 0.232 | 0.138 |
0.231 | |||
15.625 pg/ml | 0.16 | 0.163 | 0.069 |
0.165 | |||
7.8125 pg/ml | 0.125 | 0.127 | 0.033 |
0.128 | |||
0 pg/ml | 0.089 | 0.094 | 0 |
0.098 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 37.1 | 1.6 | 4.4 |
Intra-assay | 2 | 20 | 116 | 3.7 | 3.2 |
Intra-assay | 3 | 20 | 331 | 12.4 | 3.8 |
Inter-assay | 1 | 20 | 36.9 | 3.9 | 10.5 |
Inter-assay | 2 | 20 | 115 | 7.8 | 6.8 |
Inter-assay | 3 | 20 | 332 | 18.9 | 5.7 |
Protocols
References
Customer reviews and Q&As