Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
600 pg/ml | 2.338 | 2.376 | 2.275 |
2.415 | |||
300 pg/ml | 1.36 | 1.308 | 1.207 |
1.256 | |||
150 pg/ml | 0.678 | 0.702 | 0.601 |
0.727 | |||
75 pg/ml | 0.402 | 0.4 | 0.299 |
0.399 | |||
37.5 pg/ml | 0.246 | 0.235 | 0.134 |
0.225 | |||
18.75 pg/ml | 0.16 | 0.168 | 0.067 |
0.177 | |||
9.375 pg/ml | 0.125 | 0.134 | 0.033 |
0.143 | |||
0 pg/ml | 0.098 | 0.101 | 0 |
0.105 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 31.9 | 1.2 | 3.8 |
Intra-assay | 2 | 20 | 72.4 | 2 | 2.8 |
Intra-assay | 3 | 20 | 329 | 8.2 | 2.5 |
Inter-assay | 1 | 20 | 32.3 | 2.6 | 8 |
Inter-assay | 2 | 20 | 73.1 | 5.1 | 7 |
Inter-assay | 3 | 20 | 331 | 23.8 | 7.2 |
Protocols
References
Customer reviews and Q&As