Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
120 pg/ml | 2.177 | 2.244 | 2.118 |
2.311 | |||
60 pg/ml | 1.356 | 1.285 | 1.159 |
1.213 | |||
30 pg/ml | 0.714 | 0.737 | 0.611 |
0.76 | |||
15 pg/ml | 0.408 | 0.437 | 0.311 |
0.466 | |||
7.5 pg/ml | 0.292 | 0.282 | 0.156 |
0.272 | |||
3.75 pg/ml | 0.214 | 0.203 | 0.077 |
0.191 | |||
1.875 pg/ml | 0.17 | 0.176 | 0.05 |
0.181 | |||
0 pg/ml | 0.122 | 0.126 | 0 |
0.129 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 10.1 | 0.93 | 9.2 |
Intra-assay | 2 | 20 | 27.3 | 2.5 | 9.3 |
Intra-assay | 3 | 20 | 63.5 | 3.1 | 5 |
Inter-assay | 1 | 20 | 10.2 | 0.99 | 9.7 |
Inter-assay | 2 | 20 | 27.1 | 2.3 | 8.5 |
Inter-assay | 3 | 20 | 63 | 4.7 | 7.4 |
Protocols
References
Customer reviews and Q&As