Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1978Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
400ng/L | 2.215 | 2.155 | 2.074 |
2.095 | |||
200ng/L | 1.208 | 1.171 | 1.09 |
1.134 | |||
100ng/L | 0.605 | 0.621 | 0.539 |
0.636 | |||
50ng/L | 0.307 | 0.324 | 0.242 |
0.341 | |||
25ng/L | 0.162 | 0.167 | 0.085 |
0.172 | |||
0ng/L | 0.076 | 0.081 | 0 |
0.087 |
Precision
We measured random samples of E1978Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 211.7 | 8.7 | 4.1 |
Intra-assay | 2 | 18 | 231.8 | 6.5 | 2.8 |
Intra-assay | 3 | 18 | 108.1 | 4.5 | 4.2 |
Protocols
References
Customer reviews and Q&As