Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0385Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
40ng/L | 1.78 | 1.723 | 1.68 |
1.665 | |||
20ng/L | 0.997 | 0.931 | 0.888 |
0.864 | |||
10ng/L | 0.603 | 0.573 | 0.531 |
0.542 | |||
5ng/L | 0.269 | 0.267 | 0.224 |
0.264 | |||
2.5ng/L | 0.129 | 0.105 | 0.063 |
0.082 | |||
0ng/L | 0.039 | 0.042 | 0 |
0.046 |
Precision
We measured random samples of E0385Ra within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 34.5 | 0.9 | 2.6 |
Intra-assay | 2 | 18 | 26.5 | 1.5 | 5.5 |
Intra-assay | 3 | 18 | 10.2 | 0.5 | 5.1 |
Protocols
References
Customer reviews and Q&As