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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0746Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.138	2.238	2.196
	2.337
60ng/ml	1.268	1.351	1.309
	1.433
30ng/ml	0.569	0.582	0.54
	0.594
15ng/ml	0.338	0.314	0.273
	0.291
7.5ng/ml	0.133	0.156	0.114
	0.179
0ng/ml	0.031	0.042	0
	0.052

We measured random samples of E0746Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	60.7	1.7	2.8
Intra-assay	2	18	37.3	1.5	4.1
Intra-assay	3	18	26.7	1.2	4.4

> Protocol Booklet

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