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Home > Product > ELISA Kit > Human Phosphorylated adenosine monophosphate activated protein kinase, AMPK ELISA Kit

Human Phosphorylated adenosine monophosphate activated protein kinase, AMPK ELISA Kit

Human Phosphorylated adenosine monophosphate activated protein kinase, AMPK ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

Phosphorylated adenosine monophosphate activated protein kinase

Code

E0746Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.28ng/ml

Detection range

0.5-200ng/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Research area

Neuroscience|Metabolism|Signal Transduction|Cancer

Target protein

AMPK

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E0746Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/ml 2.138 2.238 2.196
2.337
60ng/ml 1.268 1.351 1.309
1.433
30ng/ml 0.569 0.582 0.54
0.594
15ng/ml 0.338 0.314 0.273
0.291
7.5ng/ml 0.133 0.156 0.114
0.179
0ng/ml 0.031 0.042 0
0.052

Precision

We measured random samples of E0746Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 60.7 1.7 2.8
Intra-assay 2 18 37.3 1.5 4.1
Intra-assay 3 18 26.7 1.2 4.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0746Hu

    $320.00

  • 96T

    E0746Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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