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Home > Product > ELISA Kit > Human Asprosin, ASPROSIN ELISA Kit

Human Asprosin, ASPROSIN ELISA Kit

Human Asprosin, ASPROSIN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.23ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.23ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

Asprosin

Code

E4095Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.23ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

Asprosin is a hormone that targets the liver to increase plasma glucose levels. Asprosin is secreted by white adipose tissue and circulates in the plasma. Asprosin acts in response to fasting and promotes blood glucose elevation by binding to the surface of hepatocytes. It promotes hepatocyte glucose release by activating the protein kinase A activity in the liver, resulting in rapid glucose release into the circulation. Asprosin and Fibrillin-1 are encoded by single FBN1 gene, they are translated and secreted as single precursor protein. Upon secretion these two proteins are separated by furin.

Research area

Signal Transduction

Target protein

ASPROSIN

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E4095Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/ml 2.04 2.137 2.103
2.235
32ng/ml 1.651 1.568 1.534
1.486
16ng/ml 0.992 0.988 0.954
0.984
8ng/ml 0.661 0.71 0.676
0.759
4ng/ml 0.469 0.465 0.431
0.461
0ng/ml 0.034 0.035 0
0.035

Precision

We measured random samples of E4095Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 21.7 1 4.4
Intra-assay 2 18 5 0.5 9.8
Intra-assay 3 18 20.4 1.5 7.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E4095Hu

    $320.00

  • 96T

    E4095Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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