Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E4095Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
64ng/ml | 2.04 | 2.137 | 2.103 |
2.235 | |||
32ng/ml | 1.651 | 1.568 | 1.534 |
1.486 | |||
16ng/ml | 0.992 | 0.988 | 0.954 |
0.984 | |||
8ng/ml | 0.661 | 0.71 | 0.676 |
0.759 | |||
4ng/ml | 0.469 | 0.465 | 0.431 |
0.461 | |||
0ng/ml | 0.034 | 0.035 | 0 |
0.035 |
Precision
We measured random samples of E4095Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 21.7 | 1 | 4.4 |
Intra-assay | 2 | 18 | 5 | 0.5 | 9.8 |
Intra-assay | 3 | 18 | 20.4 | 1.5 | 7.3 |
Protocols
References
Customer reviews and Q&As