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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0093Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200pg/ml	2.096	2.098	2.064
	2.099
600pg/ml	1.434	1.373	1.339
	1.311
300pg/ml	0.553	0.67	0.636
	0.787
150pg/ml	0.33	0.351	0.318
	0.372
75pg/ml	0.189	0.172	0.138
	0.154
0pg/ml	0.052	0.033	0
	0.014

We measured random samples of E0093Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	575.1	17	3
Intra-assay	2	18	458.8	24.4	5.3
Intra-assay	3	18	258.6	13.1	5.1

> Protocol Booklet

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