Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0093Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200pg/ml | 2.096 | 2.098 | 2.064 |
| 2.099 | |||
| 600pg/ml | 1.434 | 1.373 | 1.339 |
| 1.311 | |||
| 300pg/ml | 0.553 | 0.67 | 0.636 |
| 0.787 | |||
| 150pg/ml | 0.33 | 0.351 | 0.318 |
| 0.372 | |||
| 75pg/ml | 0.189 | 0.172 | 0.138 |
| 0.154 | |||
| 0pg/ml | 0.052 | 0.033 | 0 |
| 0.014 |
Precision
We measured random samples of E0093Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 575.1 | 17 | 3 |
| Intra-assay | 2 | 18 | 458.8 | 24.4 | 5.3 |
| Intra-assay | 3 | 18 | 258.6 | 13.1 | 5.1 |
Protocols
References
Customer reviews and Q&As
