Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0174Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
120ng/ml | 2.389 | 2.386 | 2.324 |
2.383 | |||
60ng/ml | 1.51 | 1.436 | 1.375 |
1.363 | |||
30ng/ml | 0.941 | 0.828 | 0.767 |
0.716 | |||
15ng/ml | 0.382 | 0.394 | 0.333 |
0.406 | |||
7.5ng/ml | 0.189 | 0.192 | 0.13 |
0.195 | |||
0ng/ml | 0.056 | 0.061 | 0 |
0.066 |
Precision
We measured random samples of E0174Rb within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 35.6 | 1.5 | 4.2 |
Intra-assay | 2 | 18 | 58.9 | 2 | 3.4 |
Intra-assay | 3 | 18 | 27 | 1.2 | 4.3 |
Protocols
References
Customer reviews and Q&As