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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1082Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.041	2.057	2.014
	2.074
3.2ng/ml	1.446	1.509	1.467
	1.573
1.6ng/ml	0.773	0.772	0.729
	0.771
0.8ng/ml	0.442	0.405	0.362
	0.369
0.4ng/ml	0.2	0.192	0.149
	0.184
0ng/ml	0.053	0.043	0
	0.032

We measured random samples of E1082Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	0.8	0	4.9
Intra-assay	2	18	4.5	0.2	5.3
Intra-assay	3	18	2.4	0.1	5.5

> Protocol Booklet

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