Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1082Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 6.4ng/ml | 2.041 | 2.057 | 2.014 |
| 2.074 | |||
| 3.2ng/ml | 1.446 | 1.509 | 1.467 |
| 1.573 | |||
| 1.6ng/ml | 0.773 | 0.772 | 0.729 |
| 0.771 | |||
| 0.8ng/ml | 0.442 | 0.405 | 0.362 |
| 0.369 | |||
| 0.4ng/ml | 0.2 | 0.192 | 0.149 |
| 0.184 | |||
| 0ng/ml | 0.053 | 0.043 | 0 |
| 0.032 |
Precision
We measured random samples of E1082Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 0.8 | 0 | 4.9 |
| Intra-assay | 2 | 18 | 4.5 | 0.2 | 5.3 |
| Intra-assay | 3 | 18 | 2.4 | 0.1 | 5.5 |
Protocols
References
Customer reviews and Q&As
