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Home > Product > ELISA Kit > Rat Choline acetyltransferase, ChAT ELISA Kit

Rat Choline acetyltransferase, ChAT ELISA Kit

Rat Choline acetyltransferase, ChAT ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Rat

Product overview

Full product name

Choline acetyltransferase

Code

E0725Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.01ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Rat

Storage

2-8°C

Assay time

1h 30m

Background

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

UniProt accession

MASS(Da)

71,864

Synonyms

Chat

Gene names

Chat

Research area

Neuroscience

Target protein

ChAT

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E0725Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 1.988 1.936 1.892
1.885
6ng/ml 0.984 0.95 0.906
0.917
3ng/ml 0.395 0.402 0.357
0.409
1.5ng/ml 0.165 0.181 0.136
0.197
0.75ng/ml 0.119 0.124 0.08
0.13
0ng/ml 0.034 0.045 0
0.056

Precision

We measured random samples of E0725Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 10.6 0.3 2.6
Intra-assay 2 18 4.3 0.2 3.7
Intra-assay 3 18 5.2 0.2 3.6

Protocols

References

Customer reviews and Q&As

No reviews
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Associated products

  • Size

    Code

    Price

  • 48T

    E0725Ra

    $320.00

  • 96T

    E0725Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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