Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1483Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 16ng/ml | 2.279 | 2.26 | 2.231 |
| 2.241 | |||
| 8ng/ml | 1.43 | 1.401 | 1.373 |
| 1.373 | |||
| 4ng/ml | 0.583 | 0.57 | 0.541 |
| 0.556 | |||
| 2ng/ml | 0.331 | 0.318 | 0.29 |
| 0.306 | |||
| 1ng/ml | 0.188 | 0.16 | 0.132 |
| 0.132 | |||
| 0ng/ml | 0.015 | 0.028 | 0 |
| 0.041 |
Precision
We measured random samples of E1483Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 8.4 | 0.3 | 3 |
| Intra-assay | 2 | 18 | 3.4 | 0.1 | 4.2 |
| Intra-assay | 3 | 18 | 6.1 | 0.2 | 3.4 |
Protocols
References
Customer reviews and Q&As
