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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0311Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.564	2.614	2.572
	2.664
16ng/ml	1.547	1.444	1.401
	1.34
8ng/ml	0.917	0.897	0.854
	0.877
4ng/ml	0.441	0.41	0.368
	0.379
2ng/ml	0.266	0.271	0.228
	0.276
0ng/ml	0.054	0.043	0
	0.031

We measured random samples of E0311Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	4.6	0.2	5.3
Intra-assay	2	18	15.1	0.5	3.6
Intra-assay	3	18	11.4	0.7	5.9

> Protocol Booklet

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