Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0311Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 32ng/ml | 2.564 | 2.614 | 2.572 |
| 2.664 | |||
| 16ng/ml | 1.547 | 1.444 | 1.401 |
| 1.34 | |||
| 8ng/ml | 0.917 | 0.897 | 0.854 |
| 0.877 | |||
| 4ng/ml | 0.441 | 0.41 | 0.368 |
| 0.379 | |||
| 2ng/ml | 0.266 | 0.271 | 0.228 |
| 0.276 | |||
| 0ng/ml | 0.054 | 0.043 | 0 |
| 0.031 |
Precision
We measured random samples of E0311Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 4.6 | 0.2 | 5.3 |
| Intra-assay | 2 | 18 | 15.1 | 0.5 | 3.6 |
| Intra-assay | 3 | 18 | 11.4 | 0.7 | 5.9 |
Protocols
References
Customer reviews and Q&As
