Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1413Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/ml | 2.112 | 2.059 | 2.03 |
| 2.006 | |||
| 800ng/ml | 1.103 | 1.047 | 1.018 |
| 0.992 | |||
| 400ng/ml | 0.422 | 0.45 | 0.421 |
| 0.479 | |||
| 200ng/ml | 0.172 | 0.176 | 0.147 |
| 0.179 | |||
| 100ng/ml | 0.081 | 0.118 | 0.088 |
| 0.155 | |||
| 0ng/ml | 0.029 | 0.029 | 0 |
| 0.03 |
Precision
We measured random samples of E1413Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 732.8 | 25 | 3.4 |
| Intra-assay | 2 | 18 | 947.8 | 24.7 | 2.6 |
| Intra-assay | 3 | 18 | 517.3 | 23.3 | 4.5 |
Protocols
References
Customer reviews and Q&As
