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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0030Mk are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600pmol/L	2.172	2.126	2.081
	2.079
800pmol/L	1.932	1.916	1.872
	1.9
400pmol/L	1.149	1.156	1.112
	1.163
200pmol/L	0.626	0.633	0.589
	0.641
100pmol/L	0.371	0.363	0.319
	0.355
0pmol/L	0.056	0.044	0
	0.032

We measured random samples of E0030Mk within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	272.6	12	4.4
Intra-assay	2	18	516.1	21.4	4.1
Intra-assay	3	18	224.2	12.1	5.4

> Protocol Booklet

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