Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0030Mk are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600pmol/L | 2.172 | 2.126 | 2.081 |
| 2.079 | |||
| 800pmol/L | 1.932 | 1.916 | 1.872 |
| 1.9 | |||
| 400pmol/L | 1.149 | 1.156 | 1.112 |
| 1.163 | |||
| 200pmol/L | 0.626 | 0.633 | 0.589 |
| 0.641 | |||
| 100pmol/L | 0.371 | 0.363 | 0.319 |
| 0.355 | |||
| 0pmol/L | 0.056 | 0.044 | 0 |
| 0.032 |
Precision
We measured random samples of E0030Mk within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 272.6 | 12 | 4.4 |
| Intra-assay | 2 | 18 | 516.1 | 21.4 | 4.1 |
| Intra-assay | 3 | 18 | 224.2 | 12.1 | 5.4 |
Protocols
References
Customer reviews and Q&As
