Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E3300Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/ml | 2.216 | 2.191 | 2.159 |
| 2.165 | |||
| 6ng/ml | 1.752 | 1.79 | 1.758 |
| 1.828 | |||
| 3ng/ml | 1.186 | 1.187 | 1.155 |
| 1.187 | |||
| 1.5ng/ml | 0.618 | 0.627 | 0.595 |
| 0.636 | |||
| 0.75ng/ml | 0.356 | 0.385 | 0.353 |
| 0.414 | |||
| 0ng/ml | 0.036 | 0.032 | 0 |
| 0.028 |
Precision
We measured random samples of E3300Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 1.3 | 0.1 | 6.1 |
| Intra-assay | 2 | 18 | 4.7 | 0.3 | 5.6 |
| Intra-assay | 3 | 18 | 2.5 | 0.1 | 5.4 |
Protocols
References
Customer reviews and Q&As
