Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1146Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
80ng/ml | 2.024 | 1.958 | 1.92 |
1.892 | |||
40ng/ml | 1.089 | 0.998 | 0.96 |
0.907 | |||
20ng/ml | 0.478 | 0.502 | 0.464 |
0.526 | |||
10ng/ml | 0.188 | 0.228 | 0.189 |
0.267 | |||
5ng/ml | 0.129 | 0.103 | 0.065 |
0.078 | |||
0ng/ml | 0.036 | 0.038 | 0 |
0.041 |
Precision
We measured random samples of E1146Ra within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 25.2 | 1.4 | 5.7 |
Intra-assay | 2 | 18 | 65.6 | 1.7 | 2.6 |
Intra-assay | 3 | 18 | 31.6 | 1.4 | 4.3 |
Protocols
References
Customer reviews and Q&As