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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0214Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.992	2.024	1.981
	2.055
1200ng/L	1.528	1.483	1.439
	1.438
600ng/L	0.581	0.608	0.565
	0.635
300ng/L	0.312	0.37	0.327
	0.429
150ng/L	0.125	0.147	0.104
	0.17
0ng/L	0.059	0.043	0
	0.028

We measured random samples of E0214Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	961.5	37.6	3.9
Intra-assay	2	18	1523.3	69.7	4.6
Intra-assay	3	18	452.5	14.6	3.2

> Protocol Booklet

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