Product overview
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Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0214Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 1.992 | 2.024 | 1.981 |
| 2.055 | |||
| 1200ng/L | 1.528 | 1.483 | 1.439 |
| 1.438 | |||
| 600ng/L | 0.581 | 0.608 | 0.565 |
| 0.635 | |||
| 300ng/L | 0.312 | 0.37 | 0.327 |
| 0.429 | |||
| 150ng/L | 0.125 | 0.147 | 0.104 |
| 0.17 | |||
| 0ng/L | 0.059 | 0.043 | 0 |
| 0.028 |
Precision
We measured random samples of E0214Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 961.5 | 37.6 | 3.9 |
| Intra-assay | 2 | 18 | 1523.3 | 69.7 | 4.6 |
| Intra-assay | 3 | 18 | 452.5 | 14.6 | 3.2 |
Protocols
References
Customer reviews and Q&As
