Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E2013Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 3200nmol/ml | 1.98 | 2.019 | 1.942 |
| 2.058 | |||
| 1600nmol/ml | 1.16 | 1.185 | 1.108 |
| 1.21 | |||
| 800nmol/ml | 0.613 | 0.597 | 0.52 |
| 0.58 | |||
| 400nmol/ml | 0.296 | 0.303 | 0.226 |
| 0.31 | |||
| 200nmol/ml | 0.159 | 0.15 | 0.073 |
| 0.141 | |||
| 0nmol/ml | 0.071 | 0.077 | 0 |
| 0.083 |
Precision
We measured random samples of E2013Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 2014.9 | 57.2 | 2.8 |
| Intra-assay | 2 | 18 | 781.1 | 33.8 | 4.3 |
| Intra-assay | 3 | 18 | 1123.2 | 45.4 | 4 |
Protocols
References
Customer reviews and Q&As
