Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0013Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 6.4ng/ml | 1.975 | 2.042 | 1.973 |
| 2.108 | |||
| 3.2ng/ml | 1.078 | 1.114 | 1.045 |
| 1.15 | |||
| 1.6ng/ml | 0.588 | 0.57 | 0.501 |
| 0.552 | |||
| 0.8ng/ml | 0.315 | 0.331 | 0.262 |
| 0.347 | |||
| 0.4ng/ml | 0.178 | 0.182 | 0.113 |
| 0.185 | |||
| 0ng/ml | 0.072 | 0.069 | 0 |
| 0.065 |
Precision
We measured random samples of E0013Bo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 4.9 | 0.1 | 2.2 |
| Intra-assay | 2 | 18 | 1.8 | 0.1 | 5.6 |
| Intra-assay | 3 | 18 | 2.4 | 0.1 | 4.4 |
Protocols
References
Customer reviews and Q&As
