Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0175Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 80ng/ml | 2.113 | 2.133 | 2.052 |
| 2.153 | |||
| 40ng/ml | 1.222 | 1.202 | 1.121 |
| 1.182 | |||
| 20ng/ml | 0.586 | 0.61 | 0.529 |
| 0.633 | |||
| 10ng/ml | 0.316 | 0.299 | 0.218 |
| 0.281 | |||
| 5ng/ml | 0.171 | 0.166 | 0.085 |
| 0.161 | |||
| 0ng/ml | 0.077 | 0.081 | 0 |
| 0.084 |
Precision
We measured random samples of E0175Rb within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 29.3 | 1.5 | 5 |
| Intra-assay | 2 | 18 | 44.1 | 1.4 | 3.1 |
| Intra-assay | 3 | 18 | 13.2 | 0.5 | 4.1 |
Protocols
References
Customer reviews and Q&As
