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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0006Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/ml	2.205	2.213	2.178
	2.221
80ng/ml	1.397	1.351	1.316
	1.306
40ng/ml	0.768	0.759	0.724
	0.751
20ng/ml	0.477	0.438	0.403
	0.398
10ng/ml	0.236	0.223	0.188
	0.209
0ng/ml	0.032	0.035	0
	0.038

We measured random samples of E0006Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	30.6	2.2	7.3
Intra-assay	2	18	91.3	2.9	3.2
Intra-assay	3	18	63.6	3.6	5.6

> Protocol Booklet

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