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Home > Product > ELISA Kit > Bovine Glutathione peroxidase, GSH-Px ELISA Kit

Bovine Glutathione peroxidase, GSH-Px ELISA Kit

Bovine Glutathione peroxidase, GSH-Px ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.58ng/ml

Detection range: 1-300ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Bovine

Product Summary

Size: 48T,96T

Sensitivity: 0.58ng/ml

Detection range: 1-300ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Bovine

Product overview

Full product name

Glutathione peroxidase

Code

E0006Bo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.58ng/ml

Detection range

1-300ng/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Bovine

Storage

2-8°C

Assay time

1h 30m

Background

Glutathione Peroxidase (GPx, EC 1.11.1.9) is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. It converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols, or reduce free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders.

Research area

Signal Transduction|Cell Biology|Metabolism

Target protein

GSH-Px

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E0006Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
160ng/ml 2.205 2.213 2.178
2.221
80ng/ml 1.397 1.351 1.316
1.306
40ng/ml 0.768 0.759 0.724
0.751
20ng/ml 0.477 0.438 0.403
0.398
10ng/ml 0.236 0.223 0.188
0.209
0ng/ml 0.032 0.035 0
0.038

Precision

We measured random samples of E0006Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 30.6 2.2 7.3
Intra-assay 2 18 91.3 2.9 3.2
Intra-assay 3 18 63.6 3.6 5.6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0006Bo

    $320.00

  • 96T

    E0006Bo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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