Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0451Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
80ug/ml | 1.981 | 2.002 | 1.914 |
2.023 | |||
40ug/ml | 1.08 | 1.113 | 1.025 |
1.146 | |||
20ug/ml | 0.588 | 0.568 | 0.48 |
0.548 | |||
10ug/ml | 0.294 | 0.31 | 0.222 |
0.325 | |||
5ug/ml | 0.168 | 0.16 | 0.072 |
0.153 | |||
0ug/ml | 0.082 | 0.088 | 0 |
0.095 |
Precision
We measured random samples of E0451Ra within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 18.4 | 0.8 | 4.2 |
Intra-assay | 2 | 18 | 48.4 | 1.1 | 2.3 |
Intra-assay | 3 | 18 | 34 | 1.7 | 4.9 |
Protocols
References
Customer reviews and Q&As