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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0007Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
500ng/L	2.252	2.267	2.215
	2.283
250ng/L	1.438	1.48	1.428
	1.522
125ng/L	0.869	0.821	0.769
	0.774
62.5ng/L	0.498	0.464	0.411
	0.429
31.25ng/L	0.289	0.281	0.229
	0.273
0ng/L	0.058	0.052	0
	0.047

We measured random samples of E0007Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	300	12.4	4.1
Intra-assay	2	18	66.3	4.1	6.2
Intra-assay	3	18	149	7.7	5.2

> Protocol Booklet

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