Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0007Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 500ng/L | 2.252 | 2.267 | 2.215 |
| 2.283 | |||
| 250ng/L | 1.438 | 1.48 | 1.428 |
| 1.522 | |||
| 125ng/L | 0.869 | 0.821 | 0.769 |
| 0.774 | |||
| 62.5ng/L | 0.498 | 0.464 | 0.411 |
| 0.429 | |||
| 31.25ng/L | 0.289 | 0.281 | 0.229 |
| 0.273 | |||
| 0ng/L | 0.058 | 0.052 | 0 |
| 0.047 |
Precision
We measured random samples of E0007Ca within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 300 | 12.4 | 4.1 |
| Intra-assay | 2 | 18 | 66.3 | 4.1 | 6.2 |
| Intra-assay | 3 | 18 | 149 | 7.7 | 5.2 |
Protocols
References
Customer reviews and Q&As
