Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E3000Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 640ng/L | 2.158 | 2.235 | 2.216 |
| 2.312 | |||
| 320ng/L | 1.237 | 1.268 | 1.249 |
| 1.299 | |||
| 160ng/L | 0.746 | 0.724 | 0.705 |
| 0.701 | |||
| 80ng/L | 0.414 | 0.41 | 0.391 |
| 0.407 | |||
| 40ng/L | 0.123 | 0.167 | 0.149 |
| 0.212 | |||
| 0ng/L | 0.023 | 0.019 | 0 |
| 0.015 |
Precision
We measured random samples of E3000Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 456.2 | 13 | 2.9 |
| Intra-assay | 2 | 18 | 99.1 | 6.7 | 6.7 |
| Intra-assay | 3 | 18 | 244.2 | 11.3 | 4.6 |
Protocols
References
Customer reviews and Q&As
