Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1441Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400pg/ml | 2.073 | 2.1 | 2.008 |
| 2.127 | |||
| 1200pg/ml | 1.224 | 1.209 | 1.117 |
| 1.194 | |||
| 600pg/ml | 0.579 | 0.566 | 0.474 |
| 0.553 | |||
| 300pg/ml | 0.322 | 0.328 | 0.236 |
| 0.333 | |||
| 150pg/ml | 0.167 | 0.175 | 0.083 |
| 0.183 | |||
| 0pg/ml | 0.087 | 0.092 | 0 |
| 0.097 |
Precision
We measured random samples of E1441Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 704.1 | 35.4 | 5 |
| Intra-assay | 2 | 18 | 2014.7 | 46 | 2.3 |
| Intra-assay | 3 | 18 | 1088.8 | 45.9 | 4.2 |
Protocols
References
Customer reviews and Q&As
