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Home > Product > ELISA Kit > Human Melatonin, MT ELISA Kit

Human Melatonin, MT ELISA Kit

Human Melatonin, MT ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.51ng/L

Detection range: 5-1000ng/L

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.51ng/L

Detection range: 5-1000ng/L

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

Melatonin

Code

E1013Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.51ng/L

Detection range

5-1000ng/L

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

Melatonin, the principal hormone of the pineal gland, is also produced by the retina, lens and GI tract. It is naturally synthesized from the amino acid tryptophan (via synthesis of serotonin) by the enzyme 5-hydroxyindole-O-methyltransferase. Production of melatonin by the pineal gland is under the influence of the suprachiasmatic nucleus of the hypothalamus (SCN -the site of a circadian clock) which receives information from the retina about the daily pattern of light and darkness. Melatonin can alter the timing of mammalian circadian rhythms, as well as regulate the reproductive alterations that occur in response to changes in day length in seasonally breeding mammals. Melatonin is also an extremely powerful antioxidant, with a particular role in the protection of nuclear and mitochondrial DNA. Therapeutically there may be many potential uses for melatonin such as in the treatment of various forms of some forms of depression, cancer, HIV, plus other viral diseases. Currently, Melatonin is a popular therapy for jet-lag and disturbances of sleep.

Research area

Neuroscience|Metabolism

Target protein

MT

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E1013Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.26 2.204 2.147
2.148
320ng/L 1.321 1.318 1.261
1.315
160ng/L 0.744 0.743 0.686
0.743
80ng/L 0.492 0.479 0.422
0.466
40ng/L 0.227 0.231 0.174
0.235
0ng/L 0.059 0.057 0
0.055

Precision

We measured random samples of E1013Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 139.6 9 6.4
Intra-assay 2 18 423 15.8 3.7
Intra-assay 3 18 278.6 16.8 6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1013Hu

    $320.00

  • 96T

    E1013Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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