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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0021Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320umol/L	2.088	2.136	2.049
	2.185
160umol/L	1.09	1.104	1.017
	1.118
80umol/L	0.558	0.575	0.488
	0.592
40umol/L	0.315	0.309	0.222
	0.303
20umol/L	0.178	0.173	0.086
	0.169
0umol/L	0.083	0.087	0
	0.091

We measured random samples of E0021Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	164.7	7.8	4.7
Intra-assay	2	18	220.8	5.8	2.6
Intra-assay	3	18	77.2	3.7	4.8

> Protocol Booklet

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