Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0021Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 320umol/L | 2.088 | 2.136 | 2.049 |
| 2.185 | |||
| 160umol/L | 1.09 | 1.104 | 1.017 |
| 1.118 | |||
| 80umol/L | 0.558 | 0.575 | 0.488 |
| 0.592 | |||
| 40umol/L | 0.315 | 0.309 | 0.222 |
| 0.303 | |||
| 20umol/L | 0.178 | 0.173 | 0.086 |
| 0.169 | |||
| 0umol/L | 0.083 | 0.087 | 0 |
| 0.091 |
Precision
We measured random samples of E0021Bo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 164.7 | 7.8 | 4.7 |
| Intra-assay | 2 | 18 | 220.8 | 5.8 | 2.6 |
| Intra-assay | 3 | 18 | 77.2 | 3.7 | 4.8 |
Protocols
References
Customer reviews and Q&As
