Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E3155Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
20nmol/L | 2.385 | 2.425 | 2.376 |
2.465 | |||
10nmol/L | 1.549 | 1.493 | 1.444 |
1.436 | |||
5nmol/L | 0.73 | 0.769 | 0.72 |
0.807 | |||
2.5nmol/L | 0.448 | 0.487 | 0.438 |
0.527 | |||
1.25nmol/L | 0.265 | 0.235 | 0.186 |
0.205 | |||
0nmol/L | 0.059 | 0.049 | 0 |
0.039 |
Precision
We measured random samples of E3155Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 6.1 | 0.3 | 5.5 |
Intra-assay | 2 | 18 | 9.6 | 0.3 | 3.2 |
Intra-assay | 3 | 18 | 4.5 | 0.3 | 6.6 |
Protocols
References
Customer reviews and Q&As