Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E3155Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 20nmol/L | 2.385 | 2.425 | 2.376 |
| 2.465 | |||
| 10nmol/L | 1.549 | 1.493 | 1.444 |
| 1.436 | |||
| 5nmol/L | 0.73 | 0.769 | 0.72 |
| 0.807 | |||
| 2.5nmol/L | 0.448 | 0.487 | 0.438 |
| 0.527 | |||
| 1.25nmol/L | 0.265 | 0.235 | 0.186 |
| 0.205 | |||
| 0nmol/L | 0.059 | 0.049 | 0 |
| 0.039 |
Precision
We measured random samples of E3155Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 6.1 | 0.3 | 5.5 |
| Intra-assay | 2 | 18 | 9.6 | 0.3 | 3.2 |
| Intra-assay | 3 | 18 | 4.5 | 0.3 | 6.6 |
Protocols
References
Customer reviews and Q&As
