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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0112Fi are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.351	2.292	2.246
	2.233
120ng/ml	1.817	1.763	1.717
	1.709
60ng/ml	1.207	1.187	1.141
	1.168
30ng/ml	0.688	0.677	0.631
	0.666
15ng/ml	0.388	0.385	0.339
	0.382
0ng/ml	0.05	0.046	0
	0.042

We measured random samples of E0112Fi within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	66.4	2.6	3.9
Intra-assay	2	18	33.6	2	6.1
Intra-assay	3	18	30.3	2.4	8

> Protocol Booklet

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