Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0112Fi are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 240ng/ml | 2.351 | 2.292 | 2.246 |
| 2.233 | |||
| 120ng/ml | 1.817 | 1.763 | 1.717 |
| 1.709 | |||
| 60ng/ml | 1.207 | 1.187 | 1.141 |
| 1.168 | |||
| 30ng/ml | 0.688 | 0.677 | 0.631 |
| 0.666 | |||
| 15ng/ml | 0.388 | 0.385 | 0.339 |
| 0.382 | |||
| 0ng/ml | 0.05 | 0.046 | 0 |
| 0.042 |
Precision
We measured random samples of E0112Fi within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 66.4 | 2.6 | 3.9 |
| Intra-assay | 2 | 18 | 33.6 | 2 | 6.1 |
| Intra-assay | 3 | 18 | 30.3 | 2.4 | 8 |
Protocols
References
Customer reviews and Q&As
