Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0989Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/L | 2.255 | 2.313 | 2.265 |
| 2.37 | |||
| 400ng/L | 1.719 | 1.821 | 1.773 |
| 1.923 | |||
| 200ng/L | 1.276 | 1.228 | 1.18 |
| 1.18 | |||
| 100ng/L | 0.688 | 0.649 | 0.601 |
| 0.61 | |||
| 50ng/L | 0.384 | 0.405 | 0.357 |
| 0.426 | |||
| 0ng/L | 0.035 | 0.048 | 0 |
| 0.061 |
Precision
We measured random samples of E0989Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 179.2 | 10.7 | 5.9 |
| Intra-assay | 2 | 18 | 261.8 | 10 | 3.8 |
| Intra-assay | 3 | 18 | 101.2 | 5.9 | 5.8 |
Protocols
References
Customer reviews and Q&As
